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αil 4 mab  (R&D Systems)


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    Structured Review

    R&D Systems αil 4 mab
    αil 4 Mab, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/%CE%B1il+4+mab/Human+IL-4+Antibody/pmc12778050-294-122-128
    Average 94 stars, based on 13 article reviews
    αil 4 mab - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Ex Vivo:

    Article Title: A distinct subset of stem-cell memory is poised for the cytotoxicity program in CD4 + T cells in humans
    Article Snippet: .. Ex vivo isolated CD4 + naïve T cells (T N ) from 3 to 6 donors were activated using αCD3/αCD28 DynaBeads Human T-Activator (Invitrogen, #11131D) for 48 hrs and cultured for 8 more days in T cell media (IMDM supplemented with 5% fetal bovine serum (FBS), 2% Human Serum) along with 40 U/mL of IL-2 cytokine (BioLegend) and 50 μg/mL of Gentamicin antibiotic (Invitrogen) along with either T H 1 (n=6 donors), T H 2 (n=6 donors) or neutral (n=3 donors) polarizing conditions; T H 1 polarizing condition: 5 ng/mL IL-12 cytokine (R&D System), 10 ng/mL IFN-γ cytokine (BD Biosciences) with 10 μg/mL αIL-17 mAb (eBio64CAP17; eBiosciences) and 5 μg/mL αIL-4 mAb (clone 3007; R&D System) and, T H 2 polarizing condition: 10 ng/mL IL-4 cytokine (R&D System) with 10 μg/mL αIL-12 mAb (clone 24910; R&D System), 10 μg/mL αIL-17 mAb (eBio64CAP17; eBiosciences) and 10 μg/mL αIFN-γ mAb (clone NIB42; BD Biosciences). ..

    Isolation:

    Article Title: A distinct subset of stem-cell memory is poised for the cytotoxicity program in CD4 + T cells in humans
    Article Snippet: .. Ex vivo isolated CD4 + naïve T cells (T N ) from 3 to 6 donors were activated using αCD3/αCD28 DynaBeads Human T-Activator (Invitrogen, #11131D) for 48 hrs and cultured for 8 more days in T cell media (IMDM supplemented with 5% fetal bovine serum (FBS), 2% Human Serum) along with 40 U/mL of IL-2 cytokine (BioLegend) and 50 μg/mL of Gentamicin antibiotic (Invitrogen) along with either T H 1 (n=6 donors), T H 2 (n=6 donors) or neutral (n=3 donors) polarizing conditions; T H 1 polarizing condition: 5 ng/mL IL-12 cytokine (R&D System), 10 ng/mL IFN-γ cytokine (BD Biosciences) with 10 μg/mL αIL-17 mAb (eBio64CAP17; eBiosciences) and 5 μg/mL αIL-4 mAb (clone 3007; R&D System) and, T H 2 polarizing condition: 10 ng/mL IL-4 cytokine (R&D System) with 10 μg/mL αIL-12 mAb (clone 24910; R&D System), 10 μg/mL αIL-17 mAb (eBio64CAP17; eBiosciences) and 10 μg/mL αIFN-γ mAb (clone NIB42; BD Biosciences). ..

    Cell Culture:

    Article Title: A distinct subset of stem-cell memory is poised for the cytotoxicity program in CD4 + T cells in humans
    Article Snippet: .. Ex vivo isolated CD4 + naïve T cells (T N ) from 3 to 6 donors were activated using αCD3/αCD28 DynaBeads Human T-Activator (Invitrogen, #11131D) for 48 hrs and cultured for 8 more days in T cell media (IMDM supplemented with 5% fetal bovine serum (FBS), 2% Human Serum) along with 40 U/mL of IL-2 cytokine (BioLegend) and 50 μg/mL of Gentamicin antibiotic (Invitrogen) along with either T H 1 (n=6 donors), T H 2 (n=6 donors) or neutral (n=3 donors) polarizing conditions; T H 1 polarizing condition: 5 ng/mL IL-12 cytokine (R&D System), 10 ng/mL IFN-γ cytokine (BD Biosciences) with 10 μg/mL αIL-17 mAb (eBio64CAP17; eBiosciences) and 5 μg/mL αIL-4 mAb (clone 3007; R&D System) and, T H 2 polarizing condition: 10 ng/mL IL-4 cytokine (R&D System) with 10 μg/mL αIL-12 mAb (clone 24910; R&D System), 10 μg/mL αIL-17 mAb (eBio64CAP17; eBiosciences) and 10 μg/mL αIFN-γ mAb (clone NIB42; BD Biosciences). ..

    Article Title: A distinct subset of stem-cell memory is poised for the cytotoxicity program in CD4 + T cells in humans
    Article Snippet: .. Ex vivo–isolated CD4 + T N or T SCM cells from three to six donors were activated using αCD3/αCD28 DynaBeads Human T-Activator (Invitrogen, no. 11131D) for 48 hours and cultured for 8 more days in T cell media ( and ; Iscove’s modified Dulbecco’s medium supplemented with 5% FBS and 2% human serum) along with IL-2 cytokine (40 U/ml; BioLegend) and gentamicin antibiotic (50 μg/ml; Invitrogen) along with either T H 1 ( n = 6 donors), T H 2 ( n = 6 donors), or neutral ( n = 3 donors) polarizing conditions [T H 1 polarizing condition: IL-12 cytokine (5 ng/ml; R&D System) and IFN-γ cytokine (10 ng/ml; BD Biosciences) with αIL-17 monoclonal antibody (mAb) (10 μg/ml; eBio64CAP17; eBiosciences) and αIL-4 mAb (5 μg/ml; clone 3007; R&D System); T H 2 polarizing condition: IL-4 cytokine (10 ng/ml; R&D System) with αIL-12 mAb (10 μg/ml; clone 24910; R&D System), αIL-17 mAb (10 μg/ml; eBio64CAP17; eBiosciences), and αIFN-γ mAb (10 μg/ml; clone NIB42; BD Biosciences). ..

    Modification:

    Article Title: A distinct subset of stem-cell memory is poised for the cytotoxicity program in CD4 + T cells in humans
    Article Snippet: .. Ex vivo–isolated CD4 + T N or T SCM cells from three to six donors were activated using αCD3/αCD28 DynaBeads Human T-Activator (Invitrogen, no. 11131D) for 48 hours and cultured for 8 more days in T cell media ( and ; Iscove’s modified Dulbecco’s medium supplemented with 5% FBS and 2% human serum) along with IL-2 cytokine (40 U/ml; BioLegend) and gentamicin antibiotic (50 μg/ml; Invitrogen) along with either T H 1 ( n = 6 donors), T H 2 ( n = 6 donors), or neutral ( n = 3 donors) polarizing conditions [T H 1 polarizing condition: IL-12 cytokine (5 ng/ml; R&D System) and IFN-γ cytokine (10 ng/ml; BD Biosciences) with αIL-17 monoclonal antibody (mAb) (10 μg/ml; eBio64CAP17; eBiosciences) and αIL-4 mAb (5 μg/ml; clone 3007; R&D System); T H 2 polarizing condition: IL-4 cytokine (10 ng/ml; R&D System) with αIL-12 mAb (10 μg/ml; clone 24910; R&D System), αIL-17 mAb (10 μg/ml; eBio64CAP17; eBiosciences), and αIFN-γ mAb (10 μg/ml; clone NIB42; BD Biosciences). ..



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